This course offers an intensive lab experience to teach students the practical and theoretical aspects of modern molecular biology. In the first part of the course, recombinant DNA methods and bioinformatics are used to clone and identify an unknown gene. In the second part of the course DNA microarray technology is used to determine the effect of environmental stress on the global gene expression program in yeast, and to identify genes that control the stress response. Weekly lectures compliment the lab sessions, covering the theory and principles underlying the experiments performed during the course.Graduate level requirements will include additional assignments, such as presenting and discussing research papers applicable to the lab projects or recent advances in molecular biology techniques. Enrollment Requirements: (MCB 181R and 181L) or MCB 184.